Journal: ACS Infectious Diseases

Article Title: Switching Bond: Generation of New Antimicrobial Peptides via the Incorporation of an Intramolecular Isopeptide Bond

doi: 10.1021/acsinfecdis.1c00037

Figure Lengend Snippet: Resistance of peptides to trypsin digestion. Percentages intact of Amp1L, Amp1EP, MSI-78, and MSIEP peptides were determined by reversed-phase HPLC comparative to the peak areas acquired at t 0 (control at 0 min set to 100% for each peak).

Article Snippet: The supernatant (50 μL) was injected onto a reversed-phase HPLC (C 18 reverse phase, Bio-Rad analytical column, 250 × 4 mm, 300 Å pore size, 7 μm particle size).

Techniques: Control

Journal: ACS Infectious Diseases

Article Title: Switching Bond: Generation of New Antimicrobial Peptides via the Incorporation of an Intramolecular Isopeptide Bond

doi: 10.1021/acsinfecdis.1c00037

Figure Lengend Snippet: Amount of % Remaining Amp1L, Amp1EP, MSI-78, and MSIEP Peptides Assessed by RP-HPLC after Incubation with 20% (v/v) Human Plasma at Different Time Points

Article Snippet: The supernatant (50 μL) was injected onto a reversed-phase HPLC (C 18 reverse phase, Bio-Rad analytical column, 250 × 4 mm, 300 Å pore size, 7 μm particle size).

Techniques: Incubation, Clinical Proteomics